FW: methyl carnoys

From:

Phyllis Davie

FW: methyl carnoys
------ Forwarded Message
From: Phyllis Davie <pdavie@phenopath.com>
Date: Wed, 12 Jun 2002 10:24:54 -0700
To: "Molinari, Betsy" <BMolinari@heart.thi.tmc.edu>
Subject: Re: methyl carnoys

Betsy,
Yes, MethylCarnoys is just Carnoys with methanol instead of ethanol.
60% methanol
30% chloroform
10% glacial acetic acid

I used it for years for immunos, but have never perfused a mouse kidney for renin! Just keep in mind that the chloroform is toxic (not to mention an anesthetic) , so you NEED to work in a well-ventilated area, especially if you are doing perfusions. A fume hood would be preferable. Also, try to avoid contact, if possible, as chloroform is absorbed directly through the skin, and gloves will be of very little protection, because the chloroform goes right through them too.

Best of luck,

Phyllis Davie
PhenoPath Laboratories--Seattle, WA
pdavie@phenopath.com

on 6/12/02 5:32 AM, Molinari, Betsy at BMolinari@heart.thi.tmc.edu wrote:

Good day everyone,

Has anyone out there heard of methyl Carnoys? I am assuming that it is Carnoys where the ethyl alcohol is replaced with methyl. It has been mentioned in a protocol that is used for perfusing mouse kidneys for renin.

Any information would be helpful and if anyone has a recipe for methyl Carnoys would be great.

Thanks in advance!

Betsy Molinari HT (ASCP)

Texas Heart Institute

Houston, Texas 77030

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