Bouins Fixation

From:louise renton

Dear All,
I need some advice please.
We are currently using Bouin's fixative to fix rat tissue samples of muscle 
and bone. The samples are approximately 1cm x 0.5cm and were fixed for 5 
days prior to infiltration with Historesin (GMA). When cut, however, the 
centres were soggy and uncutable. Is this a problem with fixation or with 
the infiltration time being insufficient or both?. I seem to remember that 
way back when when I was learning about such, that Bouins has poor 
penetration, but excellent cytological preservation. What are the comments 
and thoughts of the forum?

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