Melissa
 
You can stain for plaques and tangles separately.  The modified methenamine
silver method (HAGA) is a simple and reliable (for a silver) technique to
stain senile plaques and diffuse amyloid in AD brain (and if you do Gomori's
methenamine silvers for fungi etc. you will have the solutions already).  We
use the Gallyas method as a silver impregnation method for neurofibrillary
tangles in AD brain and this gives excellent contrast with no background
staining (but is a bit more complex and uses slightly more exotic
solutions).  I would use IHC for beta-Amyloid and PHF-Tau (AT8) by
preference.
 
Bill McMeekin 
Senior Chief Biomedical Scientist 
Neuropathology 
Newcastle General Hospital 
UK
-----Original Message-----
From: Melissa Jensen [mailto:melzy@indytel.com]
Sent: 13 June 2002 23:49
To: histonet@pathology.swmed.edu; Joan Yonchek
Subject: Re: Alzeheimers stain



Thanks much Joan! I am wondering now if there is a different silver method
to use.The reagents for Bielsowskys are numerous.Not sure I want to spend
that kind of money for a stain we may only use occasionally.Any
recommendations you have would be appreciated!

----- Original Message ----- 
From: Joan Yonchek   
To: 'Melissa Jensen'   
Sent: Thursday, June 13, 2002 4:38 PM
Subject: RE: Alzeheimers stain

Melissa,
 
Just sent the fax.  If you try the procedure let me know how it turns out.
 
Good luck,
 
Joan
Regeneration Technologies, Inc
Alachua, FL  32615

-----Original Message-----
From: Melissa Jensen [mailto:melzy@indytel.com]
Sent: Wednesday, June 12, 2002 6:33 PM
To: Joan Yonchek
Subject: Re: Alzeheimers stain


That would be great! Fax is 319-366-6976

----- Original Message ----- 
From: Joan   Yonchek 
To: 'Melissa Jensen'   
Sent: Tuesday, June 11, 2002 7:59 AM
Subject: RE: Alzeheimers stain

Melissa,
 
Microwave Cookbook for Microscopists
Art and Science of Visualization
By  Kok and Boon
 
Chapter 16, page 250
Procedure 16.15  Microwave-Stimulated King Silver Staining for
paraffin-embedded tissues.
Results:  Neurofibullary tangles and senile plaques are black.
The procedure states:  The core of the plaque is difficult to stain.  The
Alzheimer's fibrils will not always be colored.  Lloyd et al. published in
1985 a microwave method. We changed their method slightly.
                        
I have never tried this procedure but would be happy to fax you a copy.
 
Joan
Regeneration Technologies, Inc
Alachua, FL  32615


 


At 6:21 PM -0500 9/6/02, Melissa Jensen wrote:

Looking for a microwave stain to demonstrate plaque in the brain.....Can ya
help me out?