iron and formalin

From:Chastity Diane Shaffer Whitaker <>

<html> Hello.  I am not a histochemist in any way, shape, nor form and so that is why I am soliciting the advise of this list -- that and because I could not find my answer in your archives.<br> <br> I am a graduate student doing work in MRI looking at affects of iron.  In one of the articles I have read, it explains that use of formalin based fixation solutions may cause iron to come out of the cells/organelles/etc.  This was in reference to the amount of iron that could be detected by Perls' Stain, but if it is true, then the loss of iron would affect my results as well.  I have noticed some groups use methacarn rather than formalin for histochemical procedures.  Are there any suggestions, articles for reference, or experience in this area that someone would like to share???<br> <br> Thanks in advance for all help provided.<br> <br> CDSW<br> <br> <br> <x-sigsep><p></x-sigsep> <div align="center"> <font size=5 color="#008000">Chastity Diane Shaffer Whitaker<br> </font><font size=4>Biophysics Graduate Student<br> The Ohio State University<br> <hr> </font>Pager: 614.220.7218<br> aim nickname: "MattChasWhitaker"<br> <br> <font face="Flat Brush" size=5>"My will shall shape my future.  Whether I fail or succeed <br> shall be no man's doing but my own.  I am the force; I can clear<br> any obstacle before me or I can be lost in the maze.  My choice; my <br> responsibility; win or lose, only I hold the key to my destiny."<br> </font><font face="Flat Brush">Elaine Maxwell<br> </font></div> </html>
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