Re: what is the name of this Haematoxylin?
|From:||Bryan Llewellyn <email@example.com>|
Mayer (1896) and Langeron (1924) both published formulae with 4g hematoxylin
in conjunction with 50g alum in a litre of fluid, but they both used a
mixture of water and glycerol (700 to 300) as the solvent. Langeron
specified 20 mL acetic acid, Mayer specified none. Both omitted the
oxidiser. The formula appears to be a blend of Langeron's 1924 formula with
his 1942 formula (what is usually meant when "Mayer's" hemalum is
If anyone can provide a reference I would like to put the formula on
StainsFile with all the rest.
see: http://netbistro.com/~bryand and select
Staining methods, then
Mordanted hematoxylin formulae, then
Alum hematoxylin solutions
Check Mayer and Langeron in particular.
----- Original Message -----
From: "Jim Manavis" <firstname.lastname@example.org>
To: "Histonet" <HistoNet@Pathology.swmed.edu>
Sent: June 24, 2001 10:11 PM
Subject: what is the name of this Haematoxylin?
> Does anyone know what the name of this haematoxylin is, please?
> Haematoxylin 2g,
> Dist water 500ml
> Ammonium or pot. alum 25g
> Citric acid 0.75g
> Chloral hydrate 37.5g
> Sodium iodate 0.15g
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