Re: Whole body autoradiography
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As an FYI, I would like to call to your attention that whole body
autoradiography on mice can be performed without any difficulty on the
Hacker-Bright OTF with large area modification. This has been done, I venture
to say, for decades on this instrument. The cost point is well under $30,000,
so anyone considering this procedure should not be misled to believe that it
involves such as massive investment.
If you'd like more information, please feel free to give us a call or send an
e-mail. Dorothy Murphy is the best person here to speak with about details of
the procedure.
Best regards,
Elfi Hacker
HACKER Instruments & Industries Inc
1-800-4-HACKER
> We looked into this last year. A Leica sales rep, a lady, gave me the name
> of a company who could whole mouse frozen sections (not autoradiography).
> After pricing Leica macrocryostat to the tune of $150,000 plus that takes
> up vast lab space, we tried finding a contract lab. Now here is the bad
> news, this would have cost us something like $20,000/mouse (probably more!)
> for serial sections. You can call Leica for information on purchasing a
> large cryostat, and reach her. She should be able to come up with a
> contract lab. Sorry, don't have her name handy.
>
> There is a group in Minnesota who tracked injected enzyme labelled cells in
> a live mouse - not sure exactly how they did this, antibody? onto CD4/CD8??
> (probably could do enzyme conj antibodies/your protocol) inject a
> substrate that lights up, a chemiluminescent endpoint, and view the whole
> body with a CCD camera. The camera was probably cheaper than big cryostat
> OR contract work, plus you could probably do more experimentation with the
> animal. It was totally elegant as is many modern technology technics! We
> are still considering a camera.
>
> Good luck.
>
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> Bozeman MT 59717-3610
>
> 406 994-6367
> 404 994-4303 (FAX)
>
>
>
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>As an FYI, I would like to call to your attention that whole body
<BR>autoradiography on mice can be performed without any difficulty on the
<BR>Hacker-Bright OTF with large area modification. This has been done, I venture
<BR>to say, for decades on this instrument. The cost point is <B><U>well under </B></U>$30,000,
<BR>so anyone considering this procedure should not be misled to believe that it
<BR>involves such as massive investment.
<BR>
<BR>If you'd like more information, please feel free to give us a call or send an
<BR>e-mail. Dorothy Murphy is the best person here to speak with about details of
<BR>the procedure.
<BR>
<BR>Best regards,
<BR>
<BR>Elfi Hacker
<BR>HACKER Instruments & Industries Inc
<BR>1-800-4-HACKER
<BR>
<BR><BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">We looked into this last year. A Leica sales rep, a lady, gave me the name
<BR>of a company who could whole mouse frozen sections (not autoradiography).
<BR>After pricing Leica macrocryostat to the tune of $150,000 plus that takes
<BR>up vast lab space, we tried finding a contract lab. Now here is the bad
<BR>news, this would have cost us something like $20,000/mouse (probably more!)
<BR>for serial sections. You can call Leica for information on purchasing a
<BR>large cryostat, and reach her. She should be able to come up with a
<BR>contract lab. Sorry, don't have her name handy.
<BR>
<BR>There is a group in Minnesota who tracked injected enzyme labelled cells in
<BR>a live mouse - not sure exactly how they did this, antibody? onto CD4/CD8??
<BR>(probably could do enzyme conj antibodies/your protocol) inject a
<BR>substrate that lights up, a chemiluminescent endpoint, and view the whole
<BR>body with a CCD camera. The camera was probably cheaper than big cryostat
<BR>OR contract work, plus you could probably do more experimentation with the
<BR>animal. It was totally elegant as is many modern technology technics! We
<BR>are still considering a camera.
<BR>
<BR>Good luck.
<BR>
<BR>
<BR>Gayle Callis
<BR>MT,HT,HTL(ASCP)
<BR>Histopathology Supervisor
<BR>Veterinary Molecular Biology
<BR>Montana State University - Bozeman
<BR>Bozeman MT 59717-3610
<BR>
<BR>406 994-6367
<BR>404 994-4303 (FAX)
<BR>
<BR>
<BR></BLOCKQUOTE>
<BR>
<BR></FONT></HTML>
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