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<font size=3>A Schiff's methylene blue will stain DNA purple to red and
RNA blue. Ref: A.J.Garvin,B.J.Hall, R.M.Brissie, and S.S. Spicer,
Cytochemical differentiation of nucleic acids with a Schiff's Methylene
Blue Sequence. J. of Histochem & Cytochem., Vol. 24 No4, April,
1976, pg587 This one uses Bouin's as a mordant , 2% schiff's
for 30 min.,running water for 5, 0.5% periodic acid for 5, stain in
1% Schiff's for 10 min, wash in running water for 5-10min. stain in
methylene blue pH 6 for 1-3 min. dif in 95% deh. clear and mount. <br>
<br>
An older method of Dr. Spicer's is in the 3rd edition of the AFIP manual
on pg 133<br>
<br>
Rena Fail, AS,HT(ASCP)<br>
SS/IHC Laboratory<br>
Medical University of SC<br>
<br>
<br>
<br>
At 02:57 PM 6/19/01 -0500, you wrote:<br>
<blockquote type=cite class=cite cite>O.k. folks, here's a new one for
you........<br>
<br>
I've got a pathologist here in the lab that has asked me if I've heard of
a methylene blue-Schiffs method that stains RNA viral inclusions in
paraffin embedded tissue. Apparently she has seen reference to it
in text that she has been looking at but has not found a protocol for
it. So, I turn it over to you Histonetters! Anyone out there
even heard of it? Perhaps even provide me with a protocol?
Any and all help will be greatly appreciated!<br>
<br>
Renee Escalona, BS, HTL(ASCP)<br>
Laboratory Supervisor<br>
Texas Veterinary Medical Diagnostic Lab<br>
College Station, TX </font></blockquote><br>
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