Re: Methylene Blue-Schiffs

From:Rena Fail <>

<html> <font size=3>A Schiff's methylene blue will stain DNA purple to red and RNA blue. Ref: A.J.Garvin,B.J.Hall, R.M.Brissie, and S.S. Spicer, Cytochemical differentiation of nucleic acids with a Schiff's Methylene Blue Sequence.  J. of Histochem & Cytochem., Vol. 24 No4, April, 1976, pg587  This one uses Bouin's  as a mordant , 2% schiff's for 30 min.,running water for 5, 0.5% periodic acid  for 5, stain in 1% Schiff's for 10 min, wash in running water for 5-10min. stain in methylene blue pH 6 for 1-3 min. dif in 95% deh. clear and mount. <br> <br> An older method of Dr. Spicer's is in the 3rd edition of the AFIP manual on pg 133<br> <br> Rena Fail, AS,HT(ASCP)<br> SS/IHC  Laboratory<br> Medical University of SC<br> <br> <br> <br> At 02:57 PM 6/19/01 -0500, you wrote:<br> <blockquote type=cite class=cite cite>O.k. folks, here's a new one for you........<br> <br> I've got a pathologist here in the lab that has asked me if I've heard of a methylene blue-Schiffs method that stains RNA viral inclusions in paraffin embedded tissue.  Apparently she has seen reference to it in text that she has been looking at but has not found a protocol for it.  So, I turn it over to you Histonetters!  Anyone out there even heard of it?  Perhaps even provide me with a protocol?  Any and all help will be greatly appreciated!<br> <br> Renee Escalona, BS, HTL(ASCP)<br> Laboratory Supervisor<br> Texas Veterinary Medical Diagnostic Lab<br> College Station, TX </font></blockquote><br> </html>
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