Re: How would you detect the Y chromosome
The Y chromosome can by detected by in situ methods. Our hospital uses the
FISH system. It's best done on fresh tissue tissue where whole nuclei can be
extracted but can be done on fixed processed wax embedded tissue sections.
Steve Machin UK
--- "Patterson, Noelle (NIDDK)" <NoelleP@intra.niddk.nih.gov> wrote: > Hello
> Our lab is embarking on some transplant studies where we would like to prove
> the origin of certain cell types. They will transplant male donor tissue
> into a female
> recipient and determine (by Y chromosome or male antigen) if donor tissue
> was able to differentiate into other tissue (like beta cells of the
> pancreas, fibroblasts, blood vessels, etc.). Primarily the work will be
> done in mice (HY antigen) or monkey (Y chromosome: Cyno's and macaques). I
> am looking for ways (read an experimental assay; the simpler the better) to
> detect "male" cells. Though our preference is histologic detection, any and
> all approaches are being considered.
> Any guidance is welcome.
> Now, I know there is room here for "off the topic" responses. Keep them to
> a minimum; but remember Good Humor is always a happy addition to my day.
> As always, "Thank You" in advance for your help.
> Noelle Patterson
> Bethesda, MD 20889-5603
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