RE: hif-alpha

From:Luis Chiriboga <>

Yes,  I am following the procedure from the literature describing HIF1-alpha
staining in brain Tissue.  The antibody we are using is from Novus (catalog
# 100-123).  Staining requires HEIR,  which we perform in a microwave oven
(45 minutes 0.01M, pH6.0 citric acid buffer).  Antibody is incubated
overnight 4C (1:800). The primary antibody is detected via Tyramide
amplification (Dako CSA kit).  I know that these conditions are excessive,
and I have had some serious reservations about the validity of the staining
in other tissues.
Just last week I came across a reference (I have in PDF format) that
describes a procedure that is significantly less harsh.  I have not tried it
as of yet. If you like I could e-mail the reference and you could try that
-----Original Message-----
From: Aneeta Patel []
Sent: Tuesday, June 26, 2001 11:26 AM
Subject: hif-alpha

hi ALL,
I was wondering if anyone has done paraffin immunostaining for hypoxia
inducing factor -alpha
if so,i would appreciate the source and the method

Luis Chiriboga Ph.D., HT (ASCP) QIHC
New York University School Of Medicine
Kaplan Comprehensive Cancer Center
Bellevue Hospital
Department Of Pathology 4W27
27th Street & First Avenue
New York, N.Y. 10016

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