RE: hif-alpha

From:Luis Chiriboga <Luis.Chiriboga@med.nyu.edu>

Yes,  I am following the procedure from the literature describing HIF1-alpha
staining in brain Tissue.  The antibody we are using is from Novus (catalog
# 100-123).  Staining requires HEIR,  which we perform in a microwave oven
(45 minutes 0.01M, pH6.0 citric acid buffer).  Antibody is incubated
overnight 4C (1:800). The primary antibody is detected via Tyramide
amplification (Dako CSA kit).  I know that these conditions are excessive,
and I have had some serious reservations about the validity of the staining
in other tissues.
Just last week I came across a reference (I have in PDF format) that
describes a procedure that is significantly less harsh.  I have not tried it
as of yet. If you like I could e-mail the reference and you could try that
procedure
Regards
Luis
-----Original Message-----
From: Aneeta Patel [mailto:apatel@usuhs.mil]
Sent: Tuesday, June 26, 2001 11:26 AM
To: Histonet@pathology.swmed.edu
Subject: hif-alpha


hi ALL,
I was wondering if anyone has done paraffin immunostaining for hypoxia
inducing factor -alpha
if so,i would appreciate the source and the method
thanks

--------------------------------------
Luis Chiriboga Ph.D., HT (ASCP) QIHC
New York University School Of Medicine
Kaplan Comprehensive Cancer Center
Bellevue Hospital
Department Of Pathology 4W27
27th Street & First Avenue
New York, N.Y. 10016





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