We routinely do a triple-fluorochrome stain on frozen sections of fixed
laryngeal muscles. The 20u sections are cut onto gel-coated coverslips
for staining. Recently we attempted this procedure on some muscles that
had been left in formalin for almost two years, and these sections never
stay affixed through the whole staining procedure. We also tried
APTS-coated coverslips with the same result. Can this new problem be the
result of overfixation? Any suggestions?