fixation for cytokine staining
|From:||Gayle Callis <firstname.lastname@example.org>|
This is to clarify fixatives mentioned.
Neutral buffered formalin either in house formulation or commercial, R&D
Systems has a recipe for cytokine work.
4% paraformaldehyde in Dulbeccos PBS (Sigma, no Ca or Mg added). We
dissolve solid paraformaldehyde in Dulbeccos PBS using heat and stirring,
never exceeding 60C temperature), final pH is approx 7 - 7.4.
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610
404 994-4303 (FAX)
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