fixation for cytokine staining

From:Gayle Callis <>

This is to clarify fixatives mentioned.

Neutral buffered formalin either in house formulation or commercial, R&D
Systems has a recipe for cytokine work. 

4% paraformaldehyde in Dulbeccos PBS (Sigma, no Ca or Mg added).  We
dissolve solid paraformaldehyde in Dulbeccos PBS using heat and stirring,
never exceeding 60C temperature), final pH is approx 7 - 7.4.

Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)

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