Using antibodies in IHC made for ELISA and RIA applications
From: | "D. Harclerode" <dharclerode@targetprotein.com> |
Greetings all,
I am looking for assistance in cleaning up ELISA antibodies for IHC use. I
am trying to track IV drug distribution in a rat model. The antibodies sold
for drug detection are not optimized for use in tissues. Most are made in
sheep with a couple made in rabbits. I have tried various blocking methods
before the antibody (T. blue, glycine and Sudan Black when it shows up) with
no improvement. The tissue show a huge crossreactivity in nuclei and many
other types of cells. Pre adsorbing the antibody with sections of rat
kidney, heart and liver has shown some improvement, but I would appreciate
any other suggestions.
Thank you,
Donna Harclerode, HT, (ASCP), HTL, QIHC
Immunohistochemist
Target Protein Technologies
5310 Nancy Ridge Dr
San Diego, CA 92191
<< Previous Message | Next Message >>