Re: humidity chambers

From:Connie McManus <>

Hi, Mary!

for my humid chamber, I remove one of the crispers from one of my
refridgerators.  I place 4 small staining dishes inside (the crisper is
deep, so I use the dishes to elevate the working area and keep the stains
out of the water).  I place approx. 1 -2 liters of water in the crisper,
which makes a water depth of approx 5 cm.  Atop the dishes, I place a metal
slide rack, inverted so I don't have to deal with the slide dividers.  I
cover the top of the crisper with Saran Wrap and seal the edges with a thin
film of petroleum jelly.  I don't like using the Saran Wrap and will some
day replace it with a plexiglass lid cut to the dimensions of my crisper. 

One other thing ---  I like to have something absorbant beneath my slides,
so I precut paper toweling into 11 inches x 3 inches strips.  I use these
strips to absorb reagent at the end of each incubation.  I simply roll the
slide on it's edge, let the reagent drain off the slide into the toweling,
then do the rinses.  Just before placing the slides to the crisper for the
next reagent, I discard the old toweling strip and replace it with a fresh

Connie McManus

At 10:32 AM 6/4/01 -0400, Georger, Mary wrote:
>Good Morning,
>Is there anyone out there that is still hand staining large volumes of
>Immunos? I am trying to locate the plastic humidity chambers that hold about
>10 slides each. If anyone has a contact could you please let me know? Also
>if anyone has a better idea let me know, I just got a request for 250 BrDU's
Connie McManus
Veterinary Diagnostics Lab
Utah State University
Logan, UT

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