Re: endogenous peroxidase
|From:||Phil Bergin <firstname.lastname@example.org>|
We use glucose oxidase blocking. Works nicely and the sections keep
Our protocol is below if you are interested.
Use 1 unit per ml glucose oxidase in your final buffer, we make up
1 litre PBS
1.8 g glucose
64 mg Na-Azide.
We just heat the buffer to 37 C in a water bath, add the glucose oxidase
for 15 min, remove from the water bath and incubate the slides for 15
> What are you using for blocking endogenous peroxidase in acetone
> fixed frozen sections?
> I have used a weak solution of H202 which is not always effective,
> too strong and I get excessive "bubbling" of the tissue. Yes, I
> know, try a strength in between, but I was wondering if there is
> something more appropriate and less aggressive.
> I look forward to a wealth of knowledge and experience, preferably
> by 9am GMT tomorrow.
> Many thanks,
> Terry Hacker,
> Medical Research Council,
> Oxfordshire, OX11 ORD
> 01235 834393 x360
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