Re: Ringer's solution
From: | "J. A. Kiernan" <jkiernan@uwo.ca> |
On Fri, 15 Jun 2001, Kinsley, David wrote:
> I have a published protocol that uses MOPS-buffered Ringer's
> solution. I have MOPS but I have never heard of Ringer's ...
Ringer's (1893) was one of the first physiological saline
solutions, used for keeping frogs' organs such as heart and
other muscles working for a few hours in isolation. Locke
improved the brew in 1895. Mammal-friendly solutions (Tyrode,
Hanks, Dulbecco etc) soon followed. All these useful liquids
pre-date by about 50-60 years the introduction of physiologically
inert (we hope) zwitterionic buffers such as HEPES and MOPS.
Your "published protocol" should contain references that
justify the designation "MOPS-buffered Ringer." If it doesn't
you should assume that this is ignorant mumbo-jumbo and use some
universally recognized pbysiological saline solution.
----------------------------------------
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan
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