Re: Rat Brain
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Hello
First we profuse our rats, First with 100ml of Normal Saline at a rate of
about 19, after that has run thur the rat we run 100 ml of 10% NBF. Next we
remove the organs which are the heart and brains. Next after the brain is
removed from the skull, we put the brain in to a container of 10% NBF. Then
next day we trim the brain section to show which part of the brain we are
looking at.(Gross cut it).So we have slices of the brain at the thickness we
want, no thicker than a nickel.
Put that section into a cassette.
Next we put the brain section that is in the cassette into K-Dicromate for
about 3-4 hours. Next take the Cassette out of the K-DiCromate and rinse
under running water for several hours at least 4 hours.
Then we put it either back into 10% NBF until we add it to our tissue
processor.
We do not use NBF on our tissue processor.
Our first station is 70% ALC,80%ALC,95%ALC,100%ALC,100%ALC,100%ALC , then
MicroClear stations, since we also don't use xylene.
I feel that how ever your processor is set up with either 10% NBF or not it
doesn't really matter it will still work.
I then take the block , after facing the block off I put it on ice, you
might have to soak you block a little longer since your is dry.
All I know is this works for us and I just finished cutting 300 guinne pig
brains this week. Our rat brains are done the same way.
I think your problem might be that the brains have not been profused.
I hope this helps.
Sandi Miller HT
USAMRICD Research
Maryland
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>Hello
<BR>First we profuse our rats, First with 100ml of Normal Saline at a rate of
<BR>about 19, after that has run thur the rat we run 100 ml of 10% NBF. Next we
<BR>remove the organs which are the heart and brains. Next after the brain is
<BR>removed from the skull, we put the brain in to a container of 10% NBF. Then
<BR>next day we trim the brain section to show which part of the brain we are
<BR>looking at.(Gross cut it).So we have slices of the brain at the thickness we
<BR>want, no thicker than a nickel.
<BR>Put that section into a cassette.
<BR>Next we put the brain section that is in the cassette into K-Dicromate for
<BR>about 3-4 hours. Next take the Cassette out of the K-DiCromate and rinse
<BR>under running water for several hours at least 4 hours.
<BR>Then we put it either back into 10% NBF until we add it to our tissue
<BR>processor.
<BR>We do not use NBF on our tissue processor.
<BR>Our first station is 70% ALC,80%ALC,95%ALC,100%ALC,100%ALC,100%ALC , then
<BR>MicroClear stations, since we also don't use xylene.
<BR>I feel that how ever your processor is set up with either 10% NBF or not it
<BR>doesn't really matter it will still work.
<BR>I then take the block , after facing the block off I put it on ice, you
<BR>might have to soak you block a little longer since your is dry.
<BR>All I know is this works for us and I just finished cutting 300 guinne pig
<BR>brains this week. Our rat brains are done the same way.
<BR>I think your problem might be that the brains have not been profused.
<BR>I hope this helps.
<BR>Sandi Miller HT
<BR>USAMRICD Research
<BR>Maryland</FONT></HTML>
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