Re: Rat Brain
|From:||Gilmor Keshet <email@example.com>|
Fix the brain by perfusion of cold 4% PFA in PB, take out the brain and
post fix it overnight in the same solution 4deg, then section the fresh
brain by the vibratom (50-400um), or let sink in Sucrose 30%/PB and then
section by sliding microtom (20-40 um).
Brain sections is stained better in floating sections, instead of slide
mounted, if antibodies are to be used.
What does you friend has to stain with?
At 05:53 PM 6/5/2001 , you wrote:
>A friend brought me a block of rat brain to cut. (I have never cut
>brain tissue before). The tissue had been formalin fixed and paraffin
>embedded. When I tried to cut it, the tissue just crumbled out of the
>block. If I used moist heat and then chilled the block, I could get a
>small ribbon,(six or seven sections) but then the tissue would
>crumble. I'm not sure it was processed properly. Does anyone have a
>good protocol for fixing and processing rat brain? Also, my friend
>would like to cut thick sections (from 40 to 100 microns thick) What do
>I need to do that? Any help would be appreciated.
>UMDNJ, Camden NJ
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