|From:||Jennifer MacDonald <firstname.lastname@example.org>|
The Journal of Histotechnology/Vol.17, No2/June 1994 had an article by a
group at Texoma Pathology Foundation (Frieda Carson et al) regarding using
Zinc Isopropyl alcoholic unbuffered formalin as a post fixative. We did
parallel studies with their procedure and were extremely pleased. The
hospital has been using it ever since. We had no problems with precipitates
Sent: Friday, June 01, 2001 7:50 AM
> I sent this a couple of weeks ago but suspect, from the lack of replies, I
> This is particularly addressed to John K., who tells us:
> "You should not move a piece of tissue from a phosphate-buffered
> fixative into a zinc-formalin, because the zinc ions will make
> insoluble zinc phosphate as soon as they bump into phosphate ions
> derived from the perfused liquid."
> For those of us that love zinc fixation, but don't have it available other
than as a post-fixative for a few hours post cut-up when it is our turn on
surgicals, what are us poor boys to do?
> I know the problem and try to minimise it with a wash beforehand, but
realise that this is less than satisfactory.
> What is the way to go in these circumstances?
> Is an alcoholic zinc a better option?
> If so, what (non-commercial) formulation?
> Terry L Marshall
> Rotherham General Hospital, Yorkshire
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