Amy,
Never used it, but here's a reference. Beware, if the tissue doesn't fix,
you must remix. Apparently for some reason it doesn't perform well when used
in a courtroom full of television cameras.

3.Ito S, Karnovsky MJ. Formaldehyde-glutaraldehyde fixatives containing
trinitro compounds. J Cell Biol.1968;39:168a.

Brett

Brett M. Connolly, Ph.D.
Merck Research Laboratories
Dept. of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075
e-mail. brett_connolly@merck.com
 

-----Original Message-----
From: Amy Porter [mailto:portera203@yahoo.com]
Sent: Thursday, June 07, 2001 11:06 AM
To: Histonet
Subject: question about fixative-help....


Has anyone every heard of Ito's fixative? We have a
researcher interested in using it for western blots on
either frozen or paraffin embedded tissue. Need a
formula or a source.  Thanks in advance,

=====
Amy S.Porter, HT(ASCP)
Michigan State University
College of Human Medicine
portera203@yahoo.com

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