eBioscience also carries pretitrated, fluorochrome-conjugated cytokine
antibodies for intracellular staining.

For product listing and protocol, please visit:

http://www.ebioscience.com/ebioscience/appls/FCI.htm
> ----------
> > From: Gayle Callis <uvsgc@montana.edu>
> > Date: Wed, 13 Jun 2001 13:54:24 -0600
> > To: histonet@pathology.swmed.edu
> > Subject: cytokine staining cultured cells
> >
> > R and D Systems has protocols for cells, or you can try Pharmingen
> > immunocytochemistry staining methods for cytokines, check out websites.
> >
> > Fixation is with formalin OR paraformaldehyde, and you need to use
saponin
> > in buffers to permeablize the cell membrane to stain for cytokines.
> >
> > Good luck
> >
> >
> > Gayle Callis
> > MT,HT,HTL(ASCP)
> > Histopathology Supervisor
> > Veterinary Molecular Biology
> > Montana State University - Bozeman
> > Bozeman MT 59717-3610
> >
> > 406 994-6367
> > 404 994-4303 (FAX)
> >
> >
>