RE: Staining of ground bone sections

From:"Dunn-Jena, Patsy Ann" <>

We have found using Goldner's that a precipitate after the Orange G that has
to be removed by rubbing prior finishing the staining.  We just use a
kimwipe and that seems to take it off.

Patsy Dunn-Jena
Indiana University School of Dentistry
Mineralized Tissue and Histology Laboratory
Indianapolis, IN

> ----------
> From: 	Renton, Lousie, Mrs
> Sent: 	Monday, June 11, 2001 9:46 AM
> To:
> Subject: 	Staining of ground bone sections
> Greetings O fountain of all Wisdom.....
> Can anyone help me out here please. I am preparing ground 
> sections of bone with titanium implants. The tissue has been 
> embedded in Technovit 7200 and ground to a thickness of 30-50 
> microns. According to the protocol I have, the sections have to be 
> incubated in 5 mins in 10% H2O2 prior to staining with Goldner's 
> modified trichrome method. The problem is this: I find that there is 
> a crystalline precipitate on the sections (all solutions are freshly 
> filtered before use), which refuses to wash out. Any ideas as to 
> what could be causing this? What is the purpose of the H2O2 rinse 
> anyway and can it be ignored?.
> Any answers?
> Many thanks 
> Louise
> Bone research Unit
> Johannesburg
> South Africa

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