RE: Staining of ground bone sections
|From:||"Dunn-Jena, Patsy Ann" <firstname.lastname@example.org>|
We have found using Goldner's that a precipitate after the Orange G that has
to be removed by rubbing prior finishing the staining. We just use a
kimwipe and that seems to take it off.
Indiana University School of Dentistry
Mineralized Tissue and Histology Laboratory
> From: Renton, Lousie, Mrs
> Sent: Monday, June 11, 2001 9:46 AM
> To: email@example.com
> Subject: Staining of ground bone sections
> Greetings O fountain of all Wisdom.....
> Can anyone help me out here please. I am preparing ground
> sections of bone with titanium implants. The tissue has been
> embedded in Technovit 7200 and ground to a thickness of 30-50
> microns. According to the protocol I have, the sections have to be
> incubated in 5 mins in 10% H2O2 prior to staining with Goldner's
> modified trichrome method. The problem is this: I find that there is
> a crystalline precipitate on the sections (all solutions are freshly
> filtered before use), which refuses to wash out. Any ideas as to
> what could be causing this? What is the purpose of the H2O2 rinse
> anyway and can it be ignored?.
> Any answers?
> Many thanks
> Bone research Unit
> South Africa
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