RE: Mounting media woes
|From:||"Horn, Hazel V" <HornHV@archildrens.org>|
DAKO makes a great mounting media for chromogens....you can coverslip AEC or
DAB with it.............It's called Faramount. We like it alot. Dries
quickly at room temp. No special drying or recoverslipping required.
> -----Original Message-----
> From: Gayle Callis [SMTP:email@example.com]
> Sent: Wednesday, June 13, 2001 2:05 PM
> To: firstname.lastname@example.org
> Subject: Mounting media woes
> Try one of the newer liquid mounting medias for chromogens sensitive to
> solvents, like BCIP/NBT, or AEC, it is supposed to work for fluorescence
> but I have not used it for that application.
> You add a drop directly onto a section coming out of water, making sure it
> covers sections, dry it overnight or in an 60C oven for 15 min. It can be
> viewed quickly with latter, but let it stand overnight for total drying,
> THEN add a glass coverslip with permanent mounting media over the top of
> this dried mounting media covering your section.
> You can do this with DAB or AEC, a joy! Fisher Scientific sells Crystal
> Mount from Biomeda,
> or you can buy this from Biogenex, Shandon, and probably others.
> Gayle Callis
> Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> Bozeman MT 59717-3610
> 406 994-6367
> 404 994-4303 (FAX)
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