DAKO makes a great mounting media for chromogens....you can coverslip AEC or
DAB with it.............It's called Faramount.    We like it alot.   Dries
quickly at room temp.   No special drying or recoverslipping required.
Hazel

> -----Original Message-----
> From:	Gayle Callis [SMTP:uvsgc@montana.edu]
> Sent:	Wednesday, June 13, 2001 2:05 PM
> To:	histonet@pathology.swmed.edu
> Subject:	Mounting media woes
> 
> Try one of the newer liquid mounting medias for chromogens sensitive to
> solvents, like BCIP/NBT, or AEC, it is supposed to work for fluorescence
> but I have not used it for that application.
> 
> You add a drop directly onto a section coming out of water, making sure it
> covers sections, dry it overnight or in an 60C oven for 15 min.  It can be
> viewed quickly with latter, but let it stand overnight for total drying,
> THEN add a glass coverslip with permanent mounting media over the top of
> this dried mounting media covering your section.   
> 
> You can do this with DAB or AEC, a joy!  Fisher Scientific sells Crystal
> Mount from Biomeda,
> or you can buy this from Biogenex, Shandon, and probably others. 
> 
> 
> Gayle Callis
> MT,HT,HTL(ASCP)
> Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> Bozeman MT 59717-3610
> 
> 406 994-6367
> 404 994-4303 (FAX)
>