blocking with acetylated bsa can help also using serum from the animal
species you are staining (staining rat tissue, put rat serum in with the
serum block which should be the host of the secondary), and i use the serum
block before the primary and before the link.
patsy

-----Original Message-----
From: Polkovitch, Debbie (PRI) [mailto:DPolkovi@prius.jnj.com]
Sent: Wednesday, June 06, 2001 7:43 AM
To: 'histonet@pathology.swmed.edu'
Subject: InSitu



Does anyone know how to prevent background collagen staining when using DAB
with an InSitu procedure? 

Thank you, 
Debbie Polkovitch 
RW Johnson, PRI 
Spring House, PA 19477-0776