i use collagen IX and X from the U of Iowa Hybridoma Bank but have not heard
of collagen XI
Patsy Ruegg

-----Original Message-----
From: Elizaha@aol.com [mailto:Elizaha@aol.com]
Sent: Saturday, June 02, 2001 8:10 PM
To: histonet@pathology.swmed.edu
Subject: Collagen XI antibody/ processing a cell suspension for paraffin
embedding.


Hi, 

   Anyone know of a scource commercial or private for an antibody against 
Collagen XI. I've looked at all the usuall "suspects". 

    Also, I saw in Dr. Carson's histo book a technique using millipore 
filters to form a container for cells in suspension. and then processing
them 
into paraffin...it said to inject the cells into the "bag"; has anyone done 
this....I would like to try it...but doesn't the needle puncture the
bag...or 
not enuf for the cells to leak out? .... do you know other ways ....I 
sometimes will embed in agar/cells, but sometimes I worry about the warm
agar 
harming the cells. This technique was for particles/cells that do not stay
in 
a pelleted form.   
    Thanks in advance, 
     Elizabeth