Protein Tags and Mice
Hi dear listers. I want to know if anybody has experience with other
protein tags aside from the FLAG-tag. I want to generate some transgenic
animals and I would need to use antibodies against the tag in whole mouse
embryos from 8.5 to 14.5 dpc. I've been looking arround, but apart from
the FLAG-tag, I've not been able to find any references using another
tag in whole embryos, just the His, HA, etc, but only in cell cultures.
All suggestions and discouragements accepted.
Jaime J. Carvajal, PhD, MRC Fellow
Section of Gene Function and Regulation
Institute of Cancer Research
Chester Beatty Laboratories
237 Fulham Road
London SW3 6JB
Phone: (207) 352 8133 ext 5548
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