Depends on what you want to see, specific mucopolysaccharides
(histochemical staining with alcian blue, various pH's), general cartilage
staining (toluidine blue works nicely),  semiquantitation of proteoglycans
without EDTA decalcification that extracts these (safranin O/Fast Green)

A good H&E, particularly Ehrlichs hematoxylin also demonstrates cartilage.


Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)