CAP question
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Many Histology labs also have the responsibility of preparing non-gyn
cytology specimens as well as their staining. Ours includes both non-
gyn as well as gyn specimens. The Cap has a question: CYP.04400 that states:
"Is there a written policy for ensuring that nongynecological specimens with
a high potential for cross-contamination are processed and stained separately
from other specimens?" We do use a separate staining system to stain non-gyns
and gyn specimens . Our autostainer has 27stations including water rinses. We
routinely filter the ea-65/50 and OG-6 before the start of the runs for the
day but that may not be enough to ensure there is no carry over. We are
experiencing a good number of contamination carry-over when we stain semen
slides for analysis. They are spray fixed, and again fixed for a minimum of
20 minutes in 70-95% alcohol before staining. I am thinking of staining
these separately, but the contamination could be in any of the solutions. I
am going to try Positively charged slides or suggest that they simply be
wright stained . However I fear that these may not be the only chance for
cross-contamination. What are other labs doing to adress this CAP
requirement? Dr. Richmond, what are your suggestions?
Thanks for any insight you can supply,
R. Brian Fischer
Histology SR.Tech
Community Hospital of the Monterey Peninsula,
PO box HH
Monterey, Ca. 93942
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<HTML><FONT FACE=arial,helvetica><FONT COLOR="#808080" SIZE=3 FAMILY="SERIF" FACE="Times New Roman" LANG="0"><B> Many Histology labs also have the responsibility of preparing non-gyn
<BR>cytology specimens as well as their staining. Ours includes both non-
<BR>gyn as well as gyn specimens. The Cap has a question: CYP.04400 that states:
<BR>"Is there a written policy for ensuring that nongynecological specimens with
<BR>a high potential for cross-contamination are processed and stained separately
<BR>from other specimens?" We do use a separate staining system to stain non-gyns
<BR>and gyn specimens . Our autostainer has 27stations including water rinses. We
<BR>routinely filter the ea-65/50 and OG-6 before the start of the runs for the
<BR>day but that may not be enough to ensure there is no carry over. We are
<BR>experiencing a good number of contamination carry-over when we stain semen
<BR>slides for analysis. They are spray fixed, and again fixed for a minimum of
<BR>20 minutes in 70-95% alcohol before staining. I am thinking of staining
<BR>these separately, but the contamination could be in any of the solutions. I
<BR>am going to try Positively charged slides or suggest that they simply be
<BR>wright stained . However I fear that these may not be the only chance for
<BR>cross-contamination. What are other labs doing to adress this CAP
<BR>requirement? Dr. Richmond, what are your suggestions?
<BR>
<BR>Thanks for any insight you can supply,
<BR>R. Brian Fischer
<BR>Histology SR.Tech
<BR>Community Hospital of the Monterey Peninsula,
<BR>PO box HH
<BR>Monterey, Ca. 93942</B></FONT></HTML>
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