Hello there,

I just joined the histonet so this will be very helpful to me.  I 
received two mice feet to embed and cut, they were fixed then I 
decalcified them with Cal-ex.  I processed them as usual and blocked 
them, but now when I tried to cut them they won't cut, there is just 
a hole in the paraffin sections where the sample is.  When I cut it 
sounds as if the bone is still a bit hard so I soaked the blocks 
again in Cal-ex to see if that would help and I haven't yet tried to 
cut them again.  Does anyone esle have any other suggestions that 
will help or is there some other trick for cutting bone this is the 
first time I have had to do it.  Thanks for any help.


Monique Tourand
University of Alberta
S.M.R.I