goat lung fixation
<< Previous Message | Next Message >>
From: | RSRICHMOND@aol.com |
To: | HistoNet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="US-ASCII" |
Carla Aiwohi at usgs.gov (not further identified) forwards a note from
Crystal George at 4 G Farms in Sedgwick County CO, with the Kountry Kids 4-H
Club:
Crystal George,a fellow member of a goat list, is studying BRD (beef
respiratory disease) [for a 4-H Club project. Carla, please explain to our
readers in other countries what a4-H Club is.]
>>Those of you who can help please e-mail me personally at jgeorge@kci.net so
we don't bother the list [Oh, let's bother the list - it's an interesting
problem.]
I am working on a 4-H project and need to preserve lung tissue. My problem is
that I will have good and bad (infected) tissue (one is light colored and the
other is dark) and when I preserve them I don't want them to get discolored
(the lighter one would turn dark). This would defeat my purpose of having
them.<<
Color can be restored, at least temporarily, to formalin fixed specimens by
placing them in 95% alcohol. Even more important than color with lung tissue
is texture and shape. It's important to fix the lung in an expanded state by
perfusing the bronchi with formalin or other fixative (as I recall, a goat
has a very long tracheobronchial tree, so this should be easier to do than it
is with human material). Lungs should be expanded as fully as possible, the
bronchus or trachea clamped, and the whole specimen allowed to sit in a large
bath of formalin (don't crowd it) for two or three days. Slices can then be
cut with a large sharp knife (such as a restaurant ham slicer), and you will
be able to see and feel very localized lesions in the expanded lung.
Bob Richmond
Samurai Pathologist
Knoxville TN
<< Previous Message | Next Message >>