goat lung fixation

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Carla Aiwohi at usgs.gov (not further identified) forwards a note from 
Crystal George at 4 G Farms in Sedgwick County CO, with the Kountry Kids 4-H 

Crystal George,a fellow member of a goat list, is studying BRD (beef 
respiratory disease) [for a 4-H Club project. Carla, please explain to our 
readers in other countries what a4-H Club is.]

>>Those of you who can help please e-mail me personally at jgeorge@kci.net so 
we don't bother the list [Oh, let's bother the list - it's an interesting 

I am working on a 4-H project and need to preserve lung tissue. My problem is 
that I will have good and bad (infected) tissue (one is light colored and the 
other is dark) and when I preserve them I don't want them to get discolored 
(the lighter one would turn dark). This would defeat my purpose of having 

Color can be restored, at least temporarily, to formalin fixed specimens by 
placing them in 95% alcohol. Even more important than color with lung tissue 
is texture and shape. It's important to fix the lung in an expanded state by 
perfusing the bronchi with formalin or other fixative (as I recall, a goat 
has a very long tracheobronchial tree, so this should be easier to do than it 
is with human material). Lungs should be expanded as fully as possible, the 
bronchus or trachea clamped, and the whole specimen allowed to sit in a large 
bath of formalin (don't crowd it) for two or three days. Slices can then be 
cut with a large sharp knife (such as a restaurant ham slicer), and you will 
be able to see and feel very localized lesions in the expanded lung.

Bob Richmond
Samurai Pathologist
Knoxville TN

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