Re: resin sections in a water bath
<< Previous Message | Next Message >>
From: | Karen <kbowden@ucsd.edu> |
To: | Peter Carnell <petercar@mindspring.com>, HistoNet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; x-mac-creator=4D4F5353; x-mac-type=54455854; charset=us-ascii |
Peter;
I would float 5 micron PMMA sections on 70% alcohol. It softens the PMMA and
allows you to brush wrinkles out of the sections if you need to.
--
Karen Bowden
kbowden@ucsd.edu
UCSD
Dept. of Orthopedics
Peter Carnell wrote:
> I'm sectioning a polymethacrylate resin and have observed that the sections
> tend to spin/move rapidly on the water bath as they unfold. I think that
> this may be a surface tension effect, but I'm not sure - has anyone looked
> into this phenomenon? I plan to use add about 1% alcohol next time I
> section to reduce the surface tension, but I'm concerned about the effect
> of the alcohol on the resin - any thoughts or suggestions on this?
>
> Thanks,
>
> Peter Carnell
> Georgia Institute of Technology
<< Previous Message | Next Message >>