Re: light microscopy photography

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From:N Kenneison <>

Why not move to the future and do away with film and go digital using
the video camera on the scope and a cheap frame grabber (something like
WIN TV card) and then you don't have to train the phot lab to do it
properly as WYSIWYG. And if you still wnat transparencies load it into
POwerpoint or Harvard Graphics and send them to soembody to make sldes
from a powerpoint presentation. 
This works very well with both light and fluorescence photomicroscopy -
we use a standard JVC single chip camera for light and a DP10 digital
Olympus for recording the  ImmunoFluorsence on renals and skin work.
This has produced somevery good photgraphs and being able save as a
named item saves all the palaver when the prints / slides come back and
what was this immuno of and at what mag etc. (Can eat up your hard disc
space but a CDR can save alot of trouble).
Also if it isn't white enough you can adjust using image nehancement to
get the picture dead right, you can also use it for quantification if
you want to.


In message <>, writes
>     I was wondering if anyone can give me advice or hints about light 
>microscopy photography? I only have limited experience with this so here is 
>my problem. I am using  Kodak Royal Gold 100 film for prints. There is always 
>a brown/tan background on the image where to me should be white (ie no 
>tissue). This is I guess because of the filter....??? but what I am trying to 
>find out is how  or is it possible to get a white background? I know it is 
>done Iv'e seen it a thousand times in publications and books.  Is there a 
>combination of filters that is required....for H&E and are these changed when 
>you want to take pictures of images with DAB as the chromagen.!????Can anyone 
>recommend a book?
>Thanks again for all the help!

N Kenneison

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