Re: light microscopy photography

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From:Jeffrey S Crews <>

Alas, it is quite difficult to get a good white or white-gray background
when using color print film for photomicrography. Use slide film, either
daylight-balanced like Ektachrome 400  or tungsten-balanced like T160.
When using daylight film, you have to use a color-balancing filter
because tungsten light is richer in red (long) wavelengths than is
daylight or a flash. I think that Olympus' filter is called a LBD2N. 
 If you have a color temperature-balancing unit like Olympus has, that is
also a great help. It will tell you when you have the light properly
balanced for your film type.
	When you find a filter-film combination that works, remember that you
have to always keep the light voltage at the same level because it will
become redder as you turn the voltage down and bluer as you turn it up.
The easy way to do this is to always shoot with the light turned all the
way up.
	When you get your slides, you can have prints made from them. The best
ones are digital prints made from scanning in the slide, and you can get
a floppy or CDR with the file on it, too. Many small photo labs now offer
this service.
	I realize that shooting a slide and then paying for a print is not the
cheapest way to go, but it does give the best quality. For quickness and
routine prints for a lab notebook, just use a Polaroid and save the slide
film for publication. If you have to do a lot of photography, get a
digital camera and stop paying for film, period. You can justify the
initial cost to the higher-ups with the vastly greater utility and
film/processing savings.
I hope that some of this made sense.

Jeffrey Crews, HTL (ASCP)
Organogenesis, Inc.

On Sun, 25 Jun 2000 12:57:23 -0400 (EDT) writes:
>     I was wondering if anyone can give me advice or hints about light 
>microscopy photography? I only have limited experience with this so 
>here is 
>my problem. I am using  Kodak Royal Gold 100 film for prints. There is 
>a brown/tan background on the image where to me should be white (ie no 
>tissue). This is I guess because of the filter....??? but what I am 
>trying to 
>find out is how  or is it possible to get a white background? I know 
>it is 
>done Iv'e seen it a thousand times in publications and books.  Is 
>there a 
>combination of filters that is required....for H&E and are these 
>changed when 
>you want to take pictures of images with DAB as the chromagen.!????Can 
>recommend a book?
>Thanks again for all the help!

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