Elizabeth,

For LM photography you need to use a film which is designed to be used for 
tungsten-illumination. Kodak gold 100 is designed for daylight illumination. 
Use something like Kodak Ektachrome 64T (tungsten) or Ektachrome 160T. these 
are slide films and prints can be made from them. For darkfield micrographs 
of FITC staining Ektachrome 400 daylight  is widely used because of it's 
sensitivity to green. for B&W photography use the same films (64T and 160T 
with a green filter for the best resolution.

A great book to get is Photography through the Microscope, by Kodak and 
available from various vendors. Try Ted Pella (www.tedpella.com), an EM 
supplier.


----Original Message Follows----
From: Elizaha@aol.com
To: histonet@pathology.swmed.edu
Subject: light microscopy photography
Date: Sun, 25 Jun 2000 12:57:23 -0400 (EDT)

Hi,
      I was wondering if anyone can give me advice or hints about light
microscopy photography? I only have limited experience with this so here is
my problem. I am using  Kodak Royal Gold 100 film for prints. There is 
always
a brown/tan background on the image where to me should be white (ie no
tissue). This is I guess because of the filter....??? but what I am trying 
to
find out is how  or is it possible to get a white background? I know it is
done Iv'e seen it a thousand times in publications and books.  Is there a
combination of filters that is required....for H&E and are these changed 
when
you want to take pictures of images with DAB as the chromagen.!????Can 
anyone
recommend a book?

Thanks again for all the help!
Elizabeth


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