Dana,
Bodian's technique won't stain degenerating myelin. I agree with John 
Kiernan that the Swank-Davenport modification of Marchi's method is, by far, 
the best method available. Although the method is designed for block 
impregnation, free-floating formalin-fixed frozen sections can be employed 
if the sections are immersed in the osmium solution on top of glass balls. 
This seems to prevent a precipitate forming on the sections. Sections are 
then mounted from water, and also xylene, although we preferred to use 
carbol-xylene-creosote for clearing as it does not make the sections so 
brittle.
This is one technique that we used the freezing microtome for.

Ronnie Houston
Cytochemistry & Molecular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
(214) 559 7744
(214) 559 7768 - fax
rhouston@tsrh.org

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