Elizabeth,
Liver acetone powder;
I don't have a reference but I just put some 'liver acetone powder' in the 
antibody solution and vortex it. I let it sit for a day and then centrifuge 
and decant the supernatent. I think I got this from a small lab manual put 
out by the ASCP back in the 1980's.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

Phone: (404) 639-3964
FAX:  (404)639-3043



----Original Message Follows----
From: Elizaha@aol.com
To: histonet@pathology.swmed.edu
Subject: acetone-extraceted liver powder /anti-luciferase
Date: Wed, 28 Jun 2000 08:01:31 -0400 (EDT)

Hi,
      Does anyone have a protocol or reference for cleaning up polyclonals
with acetone-extracted liver powder?

Also, just for anyones information. a goat-polyclonal anti-Luciferase
(firefly) works well on ethanol fixed paraffin embedded cell
pellets,,,,haven't tried the fromalin yet.The antibody is from Promega.

  Elizabeth









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