Re: RNA Later

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From:Shelley Sheridan <>
To:HistoNet Server <>
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<BR> <P>We have used RNA Later to preserve tissue RNA.  The ideal way to preserve the RNA is by quick freezing in liquid nitrogen, but if that is not possible the RNA Later works well as long as the tissue is small or cut into small pieces and it is kept at 4 degrees for no more than a week.  If you need to store the tissue for longer time I would recommend freezing it.  We have only done RNA extraction from the tissue and we have not experimented with in situ hybridization.  I would recommend trying it out and comparing your RNA yield with other forms of RNA preservation to see if it is adequate for your needs.  We order ours from Ambion and talking to an Ambion representative would probably answer most of your questions.  </P><BR> <P> </P><BR><BR><P>Shelley K. Sheridan<BR>Research Specialist<BR>Center for Neurodegenerative Disease Research<BR>University of Pennsylvania Medical School<BR>Philadelphia, PA 19104<BR>Phone:(215)614-0051 Fax: (215) 349-5909</P><p><br><hr size=1><b>Do You Yahoo!?</b><br> Send instant messages with <a href="">Yahoo! Messenger</a>.
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