Re: Eosin to Identify Tissues

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From:Sharon Osborn <topper2u@earthlink.net>
To:d-johnson@mail.holyname.org
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Mercurochrome contains heavy metals.  Eosin is dye content.  It provides the
visibility for small specimens particularly and does not interfere with
staining.  Specimens tend to become "transparent" during processing creating
some difficulty in differentiation from the paraffin material depending upon
type of parafin/polymer being used.  The eosin does not interfere with any
staining methods used.  sharon osborn

d-johnson@mail.holyname.org wrote:

> Hello all:
> Has anyone tried a small amount of eosin (i.e. approx. 1cc in the first
> alcohol bath of the tissue processor) to mark tissues so they are easily
> seen in the block?  What is the "down side" of this practice? How does is
> differ from using Mercurochrome?
>
> Thank you very much.
>
> Deborah L. Johnson, MT(ASCP)SH, CLDir
> Administrative Director, Laboratories
> Holy Name Hospital
> Teaneck, NJ




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