Hi,
    Eosin works nicely to visualize the tissue, especially in small core
biopsies. It beats Mercurochrome because you dont have the nasty mercury
hazards to deal with.
    I dont think it is necessary to bother with exact measurement of dye
to alcohol (don't over do it of course) since the dye will wash out in
the re-hydration of the sections following deparaffinization.
Amos Brooks

d-johnson@mail.holyname.org wrote:

> Hello all:
> Has anyone tried a small amount of eosin (i.e. approx. 1cc in the first
> alcohol bath of the tissue processor) to mark tissues so they are easily
> seen in the block?  What is the "down side" of this practice? How does is
> differ from using Mercurochrome?
>
> Thank you very much.
>
> Deborah L. Johnson, MT(ASCP)SH, CLDir
> Administrative Director, Laboratories
> Holy Name Hospital
> Teaneck, NJ