Liam,

Currently I'm using the cyclin D-1 from Biocare Medical, and liking it a lot.  It's not perfect, but it's the best of the 6 clones I've tried so far.  We pretreat in 0.5M Tris, pH 10.0  in a vegetable steamer for 40 minutes at „ 90šC, followed by a 20 minute cooldown.  The extended time really made a big difference for us.  Here is the ordering info:

Cyclin D1,      cat# CM-090D       clone: DR1BC2
Biocare Medical     2940 Camino Diablo, Suite 110      Walnut Creek, CA 94596
phone  (800)799-9499     or (888)531-8287         fax (925)944-7888
website:  www.biocare.net

Good Luck,

Phyllis

  

>
>	Histonetters-   Has anyone out there got a reliable, method for
>the demonstration of Cyclin D1 in human tissues. We currently use the
>DAKO  monoclonal Cyclin D1 antibody raised in mouse, following heat
>mediated antigen retrieval in citrate buffer pH 6.0 and we are currently
>getting variable results. We have also tried a high pH(9.8-9.9)
>retrieval solution from DAKO, but with similar results.  All
>contributions welcome.
>
>			Liam Brennan
>			Histopathology Department
>			Belfast City Hospital
>			Northern Ireland
>