From: | DDittus787@aol.com |
To: | Liam.Brennan@bll.n-i.nhs.uk, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
We use Zymed Cyclin D1 - we microwave in edta buffer, zymeds ph 10 and use their diluent, high for 5 min 30% power 11.30 min, cool 15 min and run , incubate 37 degrees for 32 min. No problems with staining. Dana