| From: | AndreaH@imclone.com |
| To: | lgibbs@u.washington.edu |
| Reply-To: | |
| Content-Type: | text/plain; charset=us-ascii |
I usually leave my unfixed cryosections at RT for 30 minutes to overnight when necessary and always can get beautiful CD31, CD4, CD8 etc signal. In my experience, they should be just fine. But as always controls are necessary to make sure. Andrea