We are using ReVeal, from BioCare, for our HIER on the Kappa/Lambda slides.
It really cleaned up the background.  The ReVeal has blockers in it.  That
may explain ours, but I don't know what is in your HIER solution.


Jennifer MacDonald
San Antonio Community Hospital
Upland, CA  91786
(909) 985-2811 ext. 4148
jmacdonald@sach.org


> ----------
> From: 	JennieLynn6176@aol.com[SMTP:JennieLynn6176@aol.com]
> Sent: 	Monday, June 12, 2000 1:04 PM
> To: 	Histonet@pathology.swmed.edu
> Subject: 	HIER on Lambda
> 
> Please whoever told me to do HIER on a Lambda stain to reduce background 
> staining (when i asked about treating with peroxide) can you get back to
> me 
> to explain why exactly that worked??????  I did a retrieval on it and it
> came 
> out beautifully, but our supervisor wants to know why before he allows us
> to 
> start treating patient tissue in this manner.  If anyone can explain the 
> concept behind this, please do.  Thanks in advance.
> 
> Jennifer Macias HT
> Forum Health
> Western Reserve Care System
> Youngstown, Ohio
>