Hi All
A few questions about immunocytochemistry.  Using parafin sections 
and mouse tissue which make of antibody is recommended for cellular 
markers as well as cytokine staining?  Any special antigen retrieval 
methods for T cell markers (specially CD3)?  We have tried citrate 
buffer pH6.  Which type of trypsin does one normally use?  Any 
recipies?  Any special tips for background blocking?
Thanking you
Marilyn.
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MRS MARILYN TYLER
SURGICAL LABORATORIES               TEL NO : +27 +21 406-6228
DEPARTMENT OF SURGERY               FAX NO : +27 +21 448-6461
UNIVERSITY OF CAPE TOWN             EMAIL  : mtyler@uctgsh1.uct.ac.za
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