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Dear Fellow Histonetters
<br>Although the response rate was low, I want to thank all of those
who did respond to my request ! Included is a synopsis of the results (Sorry
for the mistakes in the original questionnaire and any that follow). I
hope this is useful to everyone !!! Thanks again!!!
<p>1) Which heating method do you use for HIER? (i.e.. Microwave, pressure
cooker, combination etc...).
<p><b>Steamer; 32%, Waterbath 13%, Microwave 29%, Pressure Cooker; 13%,
Microwave-pressure Cooker 6%.</b>
<p>2) What is the average "real" heating time for antigen retrieval? (actual
boiling time).
<p><b>0-5 minutes; 13%, 5-10 minutes; 13%, 10-20 minutes; 35%, 20-30
minutes; 16%, 30+ minutes; 10%</b>
<p>3) How long is your cooling period?
<p><b>0-5 minutes; 13%, 5-10 minutes; 19%, 10-20 minutes; 42%, 20-30
minutes; 13%, 30+ minutes; 6%</b>
<p>4) Do you perform manual or automated IHC?
<p><b>Manual; 32%, Automated; 42%, Both; 16%.</b>
<p>5) Do you prepare your own retrieval solutions or purchase commercial?
<p><b>Commercial; 61%, In-house; 29%, Both 6%.</b>
<p>6) Do you use more than one HIER solution? (please list)
<p><b>Yes; 48%, No; 45%</b>
<p>Retrieval Solutions Used (In alphabetical order)
<br><b>1% Zinc Sulfate</b>
<br><b>Antigen Decloaker</b>
<br><b>Biocare EDTA</b>
<br><b>Biocare Reveal</b>
<br><b>Biogenex-AR-10</b>
<br><b>Biogenex-Glyca</b>
<br><b>Biogenex Citra</b>
<br><b>Citric Acid pH6.0 NOS</b>
<br><b>Dako Citrate Buffer</b>
<br><b>Dako Target Retrieval</b>
<br><b>EDTA pH10.0 NOS</b>
<br><b>EDTA pH7.0 NOS</b>
<br><b>EDTA pH8.0 NOS</b>
<br><b>Herceptest Retrieval Buffer</b>
<br><b>Nuclear DeCloaker</b>
<br><b>Vector Ag Retrieval Solution</b>
<br><b>Zymed Citrate pH6.0</b>
<p>7) Which solution do you use the most?
<p><b>Most frequently used retrieval solution Citric Acid pH6.0 (Homemade);
26%,</b>
<br>
<p>
<br>--
<br>Luis Chiriboga Ph.D HT(ASCP)QIHC
<br>Department of Pathology 4W27
<br>Bellevue Hospital
<br>NYU School of Medicine
<br>First Avenue & 27th Street
<br>New York, NY 10016
<br>(212)562-4667
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