Gram Staining

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From:Trish Mahoney <>
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Has anyone applied a Gram stain protocol to tissue processed in Technovit
(Resin) or frozen tissue (5% Paraformaldehyde/PBS Buffer in 1M Sucrose);
and if so how successful was the protocol?
Many thanks
Trish Mahoney

Trish Mahoney,
PhD student,
Department of Anatomy and Structural Biology,
School of Medical Sciences
University of Otago
PO Box 913,

e-mail =
phone = 03. 479-7351
Room 314 Lindo Ferguson Building 

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