unfixed cryo sections left at room temp...
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From: | "L. Gibbs" <lgibbs@u.washington.edu> |
To: | Histonet <HistoNet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
Hi Histonetters-
When I do cryosectioning on unfixed specimens, I usually let
sections air-dry for a couple minutes and then store at -80C
until needed. For most applications we would then remove slides
from freezer and allow condensation to evaporate before doing a
post-fix. On occasion, I have done ICC on slides without
fixation.
More often, unfixed cryosections are cut then post-fixed within a
few minutes and air-dried. These fixed slides may be stored at
room temperature for a day or at 4C or back to the freezer for
long-term storage.
The question:
I just received some un-fixed cryo slides that have been at
r.t. for 24 hours.
They are for cytokeratin (various antibodies) ICC.
How long can unfixed cyro-sections be left at r.t. before the
tissue and epitope integrity are jeopardized?
Thanks much!
Lorraine Gibbs
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