hydrogen peroxide treatment on IHC

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We currently treat some of our slides, such as kappa and B and T cell 
markers, with hydrogen peroxide prior to staining to reduce the background 
staining that we were getting.  If anyone else does this, do you know if 
there is a time limit that you should not exceed in the hydrogen peroxide?  
Currently we are immersing 10-15 minutes in the hydrogen peroxide before 
loading onto a Ventana automated stainer.  I wanted to know if an increase in 
time would  #1-reduce more of the background, #2- just be a waste of time, or 
#3 would damage the tissue and results.  Thanks in advance for a response.

Jennifer Macias, HT (ASCP)
Forum Health
Western Reserve Care System
Youngstown, Ohio

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