hydrogen peroxide treatment on IHC
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From: | JennieLynn6176@aol.com |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="US-ASCII" |
We currently treat some of our slides, such as kappa and B and T cell
markers, with hydrogen peroxide prior to staining to reduce the background
staining that we were getting. If anyone else does this, do you know if
there is a time limit that you should not exceed in the hydrogen peroxide?
Currently we are immersing 10-15 minutes in the hydrogen peroxide before
loading onto a Ventana automated stainer. I wanted to know if an increase in
time would #1-reduce more of the background, #2- just be a waste of time, or
#3 would damage the tissue and results. Thanks in advance for a response.
Jennifer Macias, HT (ASCP)
Forum Health
Western Reserve Care System
Youngstown, Ohio
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