antibody staining for beta-gal
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From: | meadows <meadows@neuro.mcg.edu> |
To: | HistoNet Server <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="ISO-8859-1" |
Hello fellow histonetters!
A while back I asked this question but I don't think I did a very good job of
it. I would like to know if anyone out there has used an antibody for
beta-galactosidase successfully. I've gotten some good reports from the
literature for a 5 prime-3 prime antibody but they are no longer in business.
I've been unsuccessful in my search to see if anyone else is currently selling
this antibody. The work will be done on frozen/paraffin mouse brain sections.
It would be best to have a polyclonal antibody under the circumstances but at
this point I just want one that will work. We've already invested quite a bit
of money on this project with no consistent antibody results yet. We will be
doing the bacterial (X-gal) staining also - thanks to the "Bible" that was so
kindly sent to me, but we also need the antibody data. Thanks for all your
help everyone!
Angeline M. Studdard (formerly Meadows)
Cell Biology and Anatomy
Medical College of Georgia
August, GA
meadows@neuro.mcg.edu
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