Vimentin staining
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From: | "Philopena, Jennifer" <jennifer.philopena@canji.com> |
To: | 'Histonet' <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="windows-1252" |
Hi. I'm trying to stain for Kuppfer cells in mouse liver using an
anti-vimentin antibody - to no avail. Frozen tissue, two different clones
from Zymed at very high and low concentrations, MeOH or acetone fixation,
trypsin or pro K or no digestion, used ABC method and fluorescence. I would
really REALLY appreciate any suggestions, using a different antibody or
different method. THANKS in advance!!!
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