Vimentin staining

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From:"Philopena, Jennifer" <>
To:'Histonet' <>
Content-Type:text/plain; charset="windows-1252"

Hi.  I'm trying to stain for Kuppfer cells in mouse liver using an
anti-vimentin antibody - to no avail.  Frozen tissue, two different clones
from Zymed at very high and low concentrations, MeOH or acetone fixation,
trypsin or pro K or no digestion, used ABC method and fluorescence.  I would
really REALLY appreciate any suggestions, using a different antibody or
different method.  THANKS in advance!!!

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