Re: proteolytic enzymes

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From:Kappeler Andreas <>
To:Histonet <>
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Hi Louise

We routinely prepare a stock solution for Pronase (Boehringer, now Roche)
and freeze it. The concentration of the stock is 10 mg/ml in TBS and it is
stored at -80 C (long term) or -20 C (to be used within 2 months) in
aliquots of 100 or 200 ul. We dilute 1:10 to get the working concentration,
which is then stored at 4 C and is to be used within 7 days. Never faced any
problems with poor performance of the enzyme.

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

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